extracellular alp staining Search Results


96
Cell Signaling Technology Inc mek inhibitor pd98059
rhPDGF-BB activated ERK pathway in diabetic BMSCs. Cells were pretreated with or without 20 μ M <t>PD98059</t> for 1 h and treated with 10 ng/mL rhPDGF-BB for the indicated periods. The unmanipulated cells were used as controls. (a) Representative image of western blotting of p-ERK, ERK, and β -actin ( n = 3). (b) The ratio of p-ERK/ERK intensity were quantified by densitometry and expressed graphically. * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.
Mek Inhibitor Pd98059, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extracellular+alp+staining/PD98059/pmc03925525-33-1-7
Average 96 stars, based on 1 article reviews
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96
Proteintech rabbit polyclonal anti piezo1

Rabbit Polyclonal Anti Piezo1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extracellular+alp+staining/Piezo1+(extracellular+domain)+Antibody/pmc10590817-8-0-4
Average 96 stars, based on 1 article reviews
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96
Beyotime extracellular alp staining

Extracellular Alp Staining, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extracellular+alp+staining/Extracellular+Solution/10__1016_slash_j__jot__2025__10__003-228-0-16
Average 96 stars, based on 1 article reviews
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93
Boster Bio rabbit anti cd44

Rabbit Anti Cd44, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extracellular+alp+staining/Anti-CD44+Rabbit+Monoclonal+Antibody/pm22886981-38-20-23
Average 93 stars, based on 1 article reviews
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96
Proteintech extracellular signal regulated protein kinase 1 2

Extracellular Signal Regulated Protein Kinase 1 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/extracellular+alp+staining/PRKX+Antibody/pm39048236-56-72-89
Average 96 stars, based on 1 article reviews
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Image Search Results


rhPDGF-BB activated ERK pathway in diabetic BMSCs. Cells were pretreated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB for the indicated periods. The unmanipulated cells were used as controls. (a) Representative image of western blotting of p-ERK, ERK, and β -actin ( n = 3). (b) The ratio of p-ERK/ERK intensity were quantified by densitometry and expressed graphically. * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.

Journal: BioMed Research International

Article Title: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway

doi: 10.1155/2014/637415

Figure Lengend Snippet: rhPDGF-BB activated ERK pathway in diabetic BMSCs. Cells were pretreated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB for the indicated periods. The unmanipulated cells were used as controls. (a) Representative image of western blotting of p-ERK, ERK, and β -actin ( n = 3). (b) The ratio of p-ERK/ERK intensity were quantified by densitometry and expressed graphically. * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.

Article Snippet: The MEK inhibitor PD98059 was purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Western Blot, Control

rhPDGF-BB-induced cell proliferation was inhibited by suppression of ERK pathway. Cells were pretreated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB. The unmanipulated cells were used as controls. MTT assay was assessed on days 2, 4, and 7. * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.

Journal: BioMed Research International

Article Title: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway

doi: 10.1155/2014/637415

Figure Lengend Snippet: rhPDGF-BB-induced cell proliferation was inhibited by suppression of ERK pathway. Cells were pretreated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB. The unmanipulated cells were used as controls. MTT assay was assessed on days 2, 4, and 7. * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.

Article Snippet: The MEK inhibitor PD98059 was purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: MTT Assay, Control

rhPDGF-BB-induced osteogenic differentiation in diabetic BMSCs was inhibited by suppression of ERK pathway. Cells were pre-treated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB. The unmanipulated cells were used as controls. (a) Real-time PCR analysis of ALP, Runx2, and OCN mRNA expression on day 7. (b) Western blot analysis for the relative Runx2 protein levels on day 7. (c) ALP staining on day 14 (16x). (d) Quantitative results of ALP assay on day 14. (e) Alizarin red staining for mineralized nodules on day 21 (50x). * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.

Journal: BioMed Research International

Article Title: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway

doi: 10.1155/2014/637415

Figure Lengend Snippet: rhPDGF-BB-induced osteogenic differentiation in diabetic BMSCs was inhibited by suppression of ERK pathway. Cells were pre-treated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB. The unmanipulated cells were used as controls. (a) Real-time PCR analysis of ALP, Runx2, and OCN mRNA expression on day 7. (b) Western blot analysis for the relative Runx2 protein levels on day 7. (c) ALP staining on day 14 (16x). (d) Quantitative results of ALP assay on day 14. (e) Alizarin red staining for mineralized nodules on day 21 (50x). * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.

Article Snippet: The MEK inhibitor PD98059 was purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Staining, ALP Assay, Control

Journal: iScience

Article Title: PPP1R3A inhibits osteogenesis and negatively regulates intracellular calcium levels in calcific tendinopathy

doi: 10.1016/j.isci.2023.107784

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Piezo1 , Proteintech , Cat# 15939-1-AP, RRID: AB_2231460.

Techniques: Virus, Recombinant, Staining, CCK-8 Assay, ALP Assay, Reporter Assay, RNA Binding Assay, Immunoprecipitation, Negative Control, Plasmid Preparation, Software, Microscopy