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Image Search Results
Journal: BioMed Research International
Article Title: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway
doi: 10.1155/2014/637415
Figure Lengend Snippet: rhPDGF-BB activated ERK pathway in diabetic BMSCs. Cells were pretreated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB for the indicated periods. The unmanipulated cells were used as controls. (a) Representative image of western blotting of p-ERK, ERK, and β -actin ( n = 3). (b) The ratio of p-ERK/ERK intensity were quantified by densitometry and expressed graphically. * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.
Article Snippet: The
Techniques: Western Blot, Control
Journal: BioMed Research International
Article Title: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway
doi: 10.1155/2014/637415
Figure Lengend Snippet: rhPDGF-BB-induced cell proliferation was inhibited by suppression of ERK pathway. Cells were pretreated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB. The unmanipulated cells were used as controls. MTT assay was assessed on days 2, 4, and 7. * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.
Article Snippet: The
Techniques: MTT Assay, Control
Journal: BioMed Research International
Article Title: rhPDGF-BB Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Stromal Cells from Streptozotocin-Induced Diabetic Rats through ERK Pathway
doi: 10.1155/2014/637415
Figure Lengend Snippet: rhPDGF-BB-induced osteogenic differentiation in diabetic BMSCs was inhibited by suppression of ERK pathway. Cells were pre-treated with or without 20 μ M PD98059 for 1 h and treated with 10 ng/mL rhPDGF-BB. The unmanipulated cells were used as controls. (a) Real-time PCR analysis of ALP, Runx2, and OCN mRNA expression on day 7. (b) Western blot analysis for the relative Runx2 protein levels on day 7. (c) ALP staining on day 14 (16x). (d) Quantitative results of ALP assay on day 14. (e) Alizarin red staining for mineralized nodules on day 21 (50x). * P < 0.05, versus control, and # P < 0.05, versus 10 ng/mL.
Article Snippet: The
Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Staining, ALP Assay, Control
Journal: iScience
Article Title: PPP1R3A inhibits osteogenesis and negatively regulates intracellular calcium levels in calcific tendinopathy
doi: 10.1016/j.isci.2023.107784
Figure Lengend Snippet:
Article Snippet:
Techniques: Virus, Recombinant, Staining, CCK-8 Assay, ALP Assay, Reporter Assay, RNA Binding Assay, Immunoprecipitation, Negative Control, Plasmid Preparation, Software, Microscopy